ATAC Library Kit for Assay for Transposase Accessible Chromatin scRNA Sequencing (ATAC-Seq) library preparation from SeekGene.
SeekOne DD ATAC-Seq libraries from as little as 50,000 cells in as little as 1 hour.
Initial cell lysis is performed with AffiEXTRACT® Single Nuclei Isolation Kit for Neuronal Samples
CAT# AFG-INV-24, Size: 20 Preparations nuclei are spun out of the lysed cell and collected, the contaminating mitochondrial DNA stays within the cell and is discarded.
SeekOne DD will load the transposase, preloaded with Illumina adaptors, simultaneously fragments and tags (tagments) open chromatin regions
The tagmented DNA is indexed and amplified via PCR with UDI Tag Primer Set, generating high quality NGS libraries that are ready to be sequenced on any Illumina, BGI or Genemid instrument.
ATAC-seq (Assay for Transposase-Accessible Chromatin using sequencing):
1. 10x Genomics
Product: Chromium Single Cell ATAC Solution.
Specialization: Offers kits and platforms for single-cell ATAC-seq, enabling high-throughput analysis of chromatin accessibility at a single-cell level.
2. Illumina
Product: Nextera DNA Flex Library Prep Kit.
Specialization: Provides tagmentation-based kits for DNA and ATAC-seq library preparation compatible with their sequencing platforms.
3. Bio-Rad
Product: Droplet-based platforms and reagents.
Specialization: Offers solutions for single-cell and bulk ATAC-seq, particularly for high-throughput projects.
4. Epicypher
Product: CUT&Tag and ATAC-seq reagents.
Specialization: Supplies tagmentation enzymes, buffers, and kits, with a focus on chromatin biology and epigenetics.
5. New England Biolabs (NEB)
Product: NEBNext® ATAC-seq Kit.
Specialization: Comprehensive kits for bulk ATAC-seq, including reagents for tagmentation, amplification, and library preparation.
6. Thermo Fisher Scientific
Product: Tn5 transposase and library prep reagents.
Specialization: Provides standalone enzymes and custom kits for tagmentation-based workflows, suitable for ATAC-seq.
7. Diagenode
Product: MicroPlex Library Preparation Kit.
Specialization: Offers kits for low-input ATAC-seq library prep, including reagents optimized for chromatin studies.
8. Takara Bio
Product: SMARTer® ATAC-seq Kit.
Specialization: Focuses on user-friendly, efficient solutions for ATAC-seq with low cell input requirements.
9. Agilent Technologies
Product: SureSelect XT Reagents.
Specialization: Supplies library prep kits and custom panels that can be adapted for ATAC-seq, along with instruments for sequencing.
10. Zymo Research
Product: D5044 Tagmentase and related buffers.
Specialization: Provides transposase enzymes and reagents for tagmentation in ATAC-seq protocols.
11. Active Motif
Product: ATAC-Seq Kit and services.
Specialization: Offers kits, reagents, and services for chromatin accessibility studies, including ATAC-seq and related epigenetic applications.